AbFlex® Recombinant Antibodies
defined antibodies for highly specific, reproducible performance
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. In addition to flexible labeling and purification options, each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
Advantages of AbFlex® Recombinant Antibodies (rAbs)
- Flexible labeling & purification options
- Consistent performance across lots
- Highly specific and sensitive antibodies
- Reliable, long-term supply
- Animal-free production
Application Data
The following data file includes application data for all of our AbFlex™ antibodies and the monoclonal antibodies from which they are based.
Application Data File.
Name | Isotype | Reactivity | Appl. | Format | Cat No. | Price | |
---|---|---|---|---|---|---|---|
AbFlex® 5-methylcytosine antibody (rAb) | IgG2a | Other (Not Species Specific) | DB | 100 µg | 91187 | $375 | Buy Now |
10 µg | 91188 | $75 | Buy Now | ||||
AbFlex® Ago 1/2/3 antibody (rAb) | IgG2a | Human | IP | 100 µg | 91217 | $375 | Buy Now |
10 µg | 91218 | $75 | Buy Now | ||||
AbFlex® AM-Tag antibody (rAb) | IgG2a | Other (Wide Range) | ChIP, ELISA, WB | 100 µg | 91111 | $375 | Buy Now |
10 µg | 91112 | $75 | Buy Now | ||||
AbFlex® AM-Tag antibody (rAb) (biotin) | IgG2a | Other (Wide Range) | ChIP, ELISA, WB | 100 µg | 91109 | $405 | Buy Now |
10 µg | 91110 | $85 | Buy Now | ||||
AbFlex® ATM phospho Ser1981 antibody (rAb) | IgG2a | Human, Mouse | DB, WB | 100 µg | 91207 | $375 | Buy Now |
10 µg | 91208 | $75 | Buy Now | ||||
AbFlex® BMI-1 antibody (rAb) | IgG2a | Human | WB | 100 µg | 91195 | $375 | Buy Now |
10 µg | 91196 | $75 | Buy Now | ||||
AbFlex® BrdU antibody (rAb) | IgG2a | Other (Wide Range) | IF | 100 µg | 91269 | $375 | Buy Now |
10 µg | 91270 | $75 | Buy Now | ||||
AbFlex® Cas9 antibody (rAb) | IgG2a | Human | ELISA, WB | 100 µg | 91123 | $375 | Buy Now |
10 µg | 91124 | $75 | Buy Now | ||||
AbFlex® Dicer antibody (rAb) | IgG2a | Mouse | WB | 100 µg | 91241 | $375 | Buy Now |
10 µg | 91242 | $75 | Buy Now | ||||
AbFlex® EED antibody (rAb) | IgG2a | Human | ChIP, ChIP-Seq, ELISA | 100 µg | 91135 | $375 | Buy Now |
10 µg | 91136 | $75 | Buy Now | ||||
AbFlex® EHMT2 (G9a) antibody (rAb) | IgG2a | Human | WB | 100 µg | 91229 | $375 | Buy Now |
10 µg | 91230 | $75 | Buy Now | ||||
AbFlex® HDAC1 antibody (rAb) | IgG2a | Human, Mouse | ChIP-Seq, WB | 100 µg | 91215 | $375 | Buy Now |
10 µg | 91216 | $75 | Buy Now | ||||
AbFlex® HDAC2 antibody (rAb) | IgG2a | Human | ChIP-Seq, WB | 100 µg | 91197 | $375 | Buy Now |
10 µg | 91198 | $75 | Buy Now | ||||
AbFlex® HDAC3 antibody (rAb) | IgG2a | Human | WB | 100 µg | 91199 | $375 | Buy Now |
10 µg | 91200 | $75 | Buy Now | ||||
AbFlex® HIF-1 alpha antibody (rAb) | IgG2a | Bovine, Human, Mouse, Rat | ELISA | 100 µg | 91139 | $375 | Buy Now |
10 µg | 91140 | $75 | Buy Now | ||||
AbFlex® His-Tag antibody (rAb) | IgG2a | Other (Not Species Specific) | WB | 100 µg | 91183 | $375 | Buy Now |
10 µg | 91184 | $75 | Buy Now | ||||
AbFlex® Histone H3K4me3 antibody (rAb) | IgG2a | Human, Other (Wide Range) | ChIP, ChIP-Seq, DB, WB | 100 µg | 91263 | $375 | Buy Now |
10 µg | 91264 | $75 | Buy Now | ||||
AbFlex® Histone H3K9me0 Antibody (rAb) | IgG2a | Human, Other (Wide Range) | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 100 µg | 91155 | $375 | Buy Now |
10 µg | 91156 | $75 | Buy Now | ||||
AbFlex® Histone H3K9ac antibody (rAb) | IgG2a | Human, Other (Wide Range) | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 100 µg | 91103 | $375 | Buy Now |
10 µg | 91104 | $75 | Buy Now | ||||
AbFlex® Histone H3K9ac antibody (rAb) (biotin) | IgG2a | Human, Other (Wide Range) | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 100 µg | 91101 | $405 | Buy Now |
10 µg | 91102 | $85 | Buy Now | ||||
AbFlex® Histone H3K27ac antibody (rAb) | IgG2a | Human | ChIP-Seq, ELISA, WB | 100 µg | 91193 | $375 | Buy Now |
10 µg | 91194 | $75 | Buy Now | ||||
AbFlex® Histone H3K56ac antibody (rAb) | IgG2a | Human, Other (Wide Range) | ChIP-Seq, ELISA | 100 µg | 91127 | $375 | Buy Now |
10 µg | 91128 | $75 | Buy Now | ||||
AbFlex® Histone H3.3 antibody (rAb) | IgG2a | Human | ChIP-Seq, WB | 100 µg | 91191 | $375 | Buy Now |
10 µg | 91192 | $75 | Buy Now | ||||
AbFlex® Histone H4K20me3 antibody (rAb) | IgG2a | Human, Other (Wide Range) | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 100 µg | 91107 | $375 | Buy Now |
10 µg | 91108 | $75 | Buy Now | ||||
AbFlex® Histone H4K20me3 antibody (rAb) (biotin) | IgG2a | Human, Other (Wide Range) | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 100 µg | 91105 | $405 | Buy Now |
10 µg | 91106 | $85 | Buy Now | ||||
AbFlex® JMJD2A antibody (rAb) | IgG2a | Human | ELISA, WB | 100 µg | 91143 | $375 | Buy Now |
10 µg | 91144 | $75 | Buy Now | ||||
AbFlex® KDM5A antibody (rAb) | IgG2a | Human | ChIP-Seq, WB | 100 µg | 91211 | $375 | Buy Now |
10 µg | 91212 | $75 | Buy Now | ||||
AbFlex® Lamin A/C antibody (rAb) | IgG2a | Human | IF, WB | 100 µg | 91189 | $375 | Buy Now |
10 µg | 91190 | $75 | Buy Now | ||||
AbFlex® MAZ antibody (rAb) | IgG2a | Human | WB | 100 µg | 91205 | $375 | Buy Now |
10 µg | 91206 | $75 | Buy Now | ||||
AbFlex® MEIS 1/2/3 antibody (rAb) | IgG2a | Human, Mouse | WB | 100 µg | 91259 | $375 | Buy Now |
10 µg | 91260 | $75 | Buy Now | ||||
AbFlex® Mili / PiwiL2 antibody (rAb) | IgG2a | Mouse | WB | 100 µg | 91253 | $375 | Buy Now |
10 µg | 91254 | $75 | Buy Now | ||||
AbFlex® MITF antibody (rAb) | IgG2a | Human | ChIP-Seq | 100 µg | 91201 | $375 | Buy Now |
10 µg | 91202 | $75 | Buy Now | ||||
AbFlex® Myc-Tag antibody (rAb) | IgG2a | Human | WB | 100 µg | 91203 | $375 | Buy Now |
10 µg | 91204 | $75 | Buy Now | ||||
AbFlex® c-Myc antibody (rAb) | IgG2a | Human | WB | 100 µg | 91213 | $375 | Buy Now |
10 µg | 91214 | $75 | Buy Now | ||||
AbFlex® Nanog antibody (rAb) | IgG2a | Mouse | WB | 100 µg | 91251 | $375 | Buy Now |
10 µg | 91252 | $75 | Buy Now | ||||
AbFlex® Notch1 antibody (rAb) | IgG2a | Human | WB | 100 µg | 91243 | $375 | Buy Now |
10 µg | 91244 | $75 | Buy Now | ||||
AbFlex® Nucleolin antibody (rAb) | IgG2a | Canine, Human | WB | 100 µg | 91231 | $375 | Buy Now |
10 µg | 91232 | $75 | Buy Now | ||||
AbFlex® p53 antibody (rAb) | IgG2a | Human | WB | 100 µg | 91247 | $375 | Buy Now |
10 µg | 91248 | $75 | Buy Now | ||||
AbFlex® PBX2 antibody (rAb) | IgG2a | Human, Mouse | WB | 100 µg | 91227 | $375 | Buy Now |
10 µg | 91228 | $75 | Buy Now | ||||
AbFlex® Pim2 antibody (rAb) | IgG2a | Human | WB | 100 µg | 91239 | $375 | Buy Now |
10 µg | 91240 | $75 | Buy Now | ||||
AbFlex® Rad21 antibody (rAb) | IgG2a | Human, Mouse | IF, WB | 100 µg | 91245 | $375 | Buy Now |
10 µg | 91246 | $75 | Buy Now | ||||
AbFlex® RNA Pol II antibody (rAb) | IgG2a | Human | ChIP-Seq, IF, WB | 100 µg | 91151 | $375 | Buy Now |
10 µg | 91152 | $75 | Buy Now | ||||
AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb) | IgG2a | Human | ELISA, IF, WB | 100 µg | 91219 | $375 | Buy Now |
10 µg | 91220 | $75 | Buy Now | ||||
AbFlex® RNA pol II CTD phospho Ser2 antibody (rAb) | IgG2a | Human | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 100 µg | 91115 | $375 | Buy Now |
10 µg | 91116 | $75 | Buy Now | ||||
AbFlex® RNA pol II CTD phospho Ser5 antibody (rAb) | IgG2a | Human | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 100 µg | 91119 | $375 | Buy Now |
10 µg | 91120 | $75 | Buy Now | ||||
AbFlex® RNA pol II CTD phospho Ser5 antibody (rAb) (biotin) | IgG2a | Human | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 100 µg | 91117 | $405 | Buy Now |
10 µg | 91118 | $85 | Buy Now | ||||
AbFlex® SMARCA1 / SNF2L1 antibody (rAb) | IgG2a | Human | WB | 100 µg | 91225 | $375 | Buy Now |
10 µg | 91226 | $75 | Buy Now | ||||
AbFlex® Snail antibody (rAb) | IgG2a | Human | WB | 100 µg | 91255 | $375 | Buy Now |
10 µg | 91256 | $75 | Buy Now | ||||
AbFlex® TET1 antibody (rAb) | IgG2a | Human, Mouse | WB | 100 µg | 91171 | $375 | Buy Now |
10 µg | 91172 | $75 | Buy Now | ||||
AbFlex® Ubiquitin antibody (rAb) | IgG2a | Other (Wide Range) | WB | 100 µg | 91257 | $375 | Buy Now |
10 µg | 91258 | $75 | Buy Now |
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

AbFlex® recombinant antibody (rAb) with flexible labeling and purification options.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

Differences in label distribution between AbFlex® direct labeling and chemical labeling.
Prior to the development of recombinant antibodies, researchers relied on polyclonal and monoclonal antibodies. Polyclonal antibodies recognize multiple epitopes on the same antigen. This increases antibody affinity, but often has a higher potential for cross-reactivity. Monoclonal antibodies represent a single antibody producing B cell that binds to one unique epitope. However, monoclonal antibodies can be lost or evolve over time.
Unlike monoclonal antibodies, which can experience gene loss, mutations and cell-line drift that alter antibody performance over time and lead to variability across antibody lots, recombinant antibodies are highly consistent because they contain the same immunoglobulin heavy and light chain DNA sequences in every lot. This provides batch-to-batch reproducibility in antibody performance and eliminates the need to re-evaluate or compare different antibody lots, thereby, saving valuable time and effort on assay development.
To ensure optimal performance, each AbFlex® antibody is carefully screened for specificity and sensitivity. Only heavy and light chain sequences that produce highly specific antibodies, as verified in our bead-based binding assay, are selected. Antibodies that utilize the AbFlex® direct labeling approach also show greater sensitivity than their parent monoclonal antibodies across a variety of applications.
Another advantage of AbFlex® recombinant antibodies is the fact that they are produced outside of an animal system. Polyclonal antibodies rely on an animal’s immune response and epitope specificity, both of which are highly variable across animals and immunizations. Polyclonal antibodies are also hindered by having a finite supply, making it difficult to perform long-term antibody projects or reproduce data over time or across labs. With AbFlex® recombinant antibodies, the full-length IgG is expressed in a mammalian system to ensure a consistent, stable antibody supply. Additionally, any ethical or animal welfare concerns associated with antibody production and maintenance are avoided.
The following list contains Active Motif's AbFlex® recombinant antibodies (rAbs). For information about site-specific labeling of the AbFlex® antibodies, please visit our collection of Sortag-IT™ Labeling Kits.