Recombinant FXR protein

Aliases: BAR, HRR1, HRR-1, RIP14, NR1H4
Catalog No: 31120 Format: 10 µg $450 Buy
Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

The farnesoid X receptor (FXR) or nuclear receptor subfamily 1, group H, member 4 (NR1H4) is a nuclear receptor that, when activated, translocates to the nucleus where it interacts with certain proteins, including RXR and PPARGC1A, forming heterodimers that bind to hormone response elements on DNA and modulate gene expression. One of the primary functions of FXR is to suppress bile acid synthesis when levels are high by inhibiting the transcription of cholesterol 7 alpha-hydroxylase (CYP7A1) gene that expresses CYP7A1, the rate-limiting enzyme in bile acid synthesis from cholesterol.

Application Notes

Recombinant FXR is suitable for DNA and protein-protein interaction assays. 20 ng is sufficient for gelshift assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~55 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Protein Details

Recombinant FXR is isolated from an E. coli strain that carries the coding sequence of the human FXR under the control of a T7 promoter (accession number U68233). The purified recombinant protein has an N-terminal His-Tag and is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.

References

This product was used in the following publications:
Biochim Biophys Acta. 2011 Mar;1809(3):157-65. doi: 10.1016/j.bbagrm.2011.01.008. Epub 2011 Feb 4.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.