Recombinant GSK3β protein

Aliases: Glycogen Synthase Kinase 3 Beta, GSK-3 Beta
Catalog No: 31173 Format: 10 µg Discontinued

Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Application Notes

Recombinant GSK3β is suitable for kinase assays and Western blot. The molecular weight of the protein is ~76.312 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF 5 µg/50 µl, Recombinant GSK3β: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

Protein Details

Recombinant human GSK3β protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-T420 , accession number L33801 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 

Recombinant GSK3β protein tested by SDS-PAGE gel.
0.5 µg of GSK3β protein was analyzed using SDS/PAGE followed by Western blot with either an anti-GSK3β or anti-GST antibody.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.