Recombinant SUV420H1 protein

Aliases: CGI85; KMT5B; CGI-85
Catalog No: 31360 Format: 50 µg Discontinued

Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Application Notes

Recombinant SUV420H1 is suitable for use in histone methyltransferase (HMT) assays. Specific Activity: 0.0009 pmol/min/μg. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Assay conditions: Prepare a 50 μl reaction mix (50 mM TRIS pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 800 μM S-adenosylmethionine, and SUV420H1) and add to substrate-coated wells. Incubate for 4 hr. Add primary antibody, incubate 1 hr. Next, incubate 30 minutes with the HRP-labeled secondary antibody followed by chemiluminescent detection.

Protein Details

SUV420H1 is a histone methyltransferase specific for dimethylation of Histone H4 at lysine 20. Amino acids 2-387 of human SUV420H1 (accession number NM_017635) were expressed in E. coli with an N-terminal GST-Tag (MW = 70 kDa).

 

SUV420H1 protein gel.
SUV420H1 run on a 4-20% SDS-PAGE gel and stained with Coomassie blue. Arrow indicates recombinant SUV420H1.

SUV420H1 activity assay.
Recombinant SUV420H1 activity measured using a fluorescent histone methyltransferase assay.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.