Recombinant KMT2D (MLL2)-SET protein

Aliases: ALR; KMS; KMT2D; MLL2; AAD10; KABUK1; TNRC21; CAGL114
Catalog No: 31420 Format: 50 µg $400 Buy
Catalog No: 31820 Format: 1 mg $3,585 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

KMT2D (MLL2)-SET (Myeloid/lymphoid or mixed-lineage leukemia 2) is a Trithorax-group protein that function collectively to promote gene expression. KMT2D (MLL2)-SET is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. KMT2D (MLL2)-SET is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the β-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

The peptide corresponding to amino acids 5355 - 5537 of the KMT2D (MLL2)-SET protein (accession number NP_003473.3) was expressed in E. coli. The peptide contains the SET domain of MLL2 that is responsible for methyltransferase activity. Recombinant KMT2D (MLL2)-SET protein contains an N-terminal GST-Tag with a molecular weight of 47.6 kDa. The recombinant protein is ≥ 80% pure by SDS-PAGE.

 

Recombinant KMT2D (MLL2)-SET protein gel.
KMT2D (MLL2)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant KMT2D (MLL2)-SET protein binding assay.
KMT2D (MLL2)-SET was incubated with equimolar other 4 components of MLL2 complex (ASH2L, RBBP5, WDR5 and DPY30) for 2 hours 4 °C.
All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.