Recombinant LSD1 / KDM1A protein

Aliases: Lysine Demethylase 1A, Flavin-Containing Amine Oxidase Domain-Containing Protein 2, Lysine-Specific Histone Demethylase 1
Catalog No: 31507 Format: 50 µg Discontinued

Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3K4me1 and Histone H3K4me2, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3K9me1 and Histone H3K9me2, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.

Application Notes

Recombinant LSD1 / KDM1A is suitable for use in histone demethylation assays. A recommended starting point for the demethylation assay is 20-100 ng of recombinant protein per reaction.

Protein Details

LSD1 (KDM1A, AOF2) is a histone demethylase that is specific to histone H3 dimethylated at lysine 4 (H3K4me2). Methylation of H3K4 is a marker of transcriptional activation. Recombinant full-length human LSD1 (GenBank Accession No. NM_015013), amino acids 158-end with N-terminal GST-Tag, was expressed in E. coli. The molecular weight of the protein is 103 kDa. The recombinant protein is >70% pure by SDS-PAGE.

 

Recombinant LSD1 / KDM1A protein gel.
LSD1 / KDM1A run on a 4-20% SDS-PAGE gel and stained with Coomassie blue.

Recombinant LSD1 / KDM1A protein activity assay.
Recombinant LSD1 / KDM1A activity measured using a fluorescent demethylase assay.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.