Recombinant SIRT6 protein

Aliases: Sirtuin 6, Regulatory Protein SIR2 Homolog, SIR2-Like Protein 6, SIRT-6
Catalog No: 31532 Format: 100 µg $450 Buy
Catalog No: 31932 Format: 1 mg $2,535 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

SIRT6 (Sir2 homologue 6) is an NAD+ dependant histone deacetylase (HDAC) related to SIRT1 and to the yeast Sir2 protein. SIRT6, originally identified as a tubulin deacetylase, was recently found to be able to deacetylate histone H3 acetylated at lysine 9.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11).

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant human SIRT6 was expressed in E. coli as the full length protein (accession number NM_016539) with an N-terminal GST-Tag. The molecular weight of the protein is ~65 kDa. The recombinant protein is > 65% pure by SDS-PAGE.

 

Recombinant SIRT6 protein run on an 10% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.