Contents
200 µg of K-562 nuclear extract at 4.3 µg/µl.
Background
K-562 nuclear extract was prepared from a cell culture of the K-562 human myelogenous leukemia cell line. The K-562 cell line was originally derived from immortalized erythroleukemia cells obtained from a patient with a Philadelphia chromosome-positive chronic myeloid leukemia in blast crisis. These pluripotent cells are widely applicable in research due to their ability to spontaneously differentiate into erythrocyte, granulocyte and monocyte progenitors. K-562 cells are also frequently utilized in in vitro natural killer (NK) cell assays as highly sensitive targets since they lack the expression of MHC class I molecules required for protection against NK activity.
Application Notes
K-562 nuclear extract is recommended for use in the studies related to 1) differentiation, 2) immunology, and 3) cancer.
Extract Origin
Human lymphoblast bone marrow; chronic myelogenous leukemia (CML)
Extract Composition
K-562 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
Quality Control
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Storage
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.