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200 µg of NIH/3T3 nuclear extract at 2.5 µg/µl.
Background
NIH/3T3 nuclear extract was prepared from a cell culture of the NIH/3T3 mouse fibroblast cell line obtained from Swiss mouse embryo tissue. This cell line was established from immortalized primary mouse embryonic fibroblast cells that were cultured by the '3T3 protocol'. 3T3 (3-day transfer, inoculum 3 x 105 cells) refers to the transfer and inoculation protocol used to initiate and propagate the cell line. In research, these cells have become the standard mouse fibroblast cell line.
Application Notes
NIH/3T3 nuclear extract is recommended for use across a wide range of applications and disciplines requiring analysis of a standard mouse fibroblast cell line.
Extract Origin
Mouse embryo
Extract Composition
NIH/3T3 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
Quality Control
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Storage
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
