c-Myc antibody (pAb) (65 kDa form)

RRID: AB_2793149
Aliases: MRTL, MYCC, c-Myc, bHLHe39
Catalog No: 39012 Format: 100 µl Discontinued

Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
65 kDa
Reactivity:
Human, Monkey, Mouse

Applications

Western Blot Validated

Application Notes


This product has been discontinued:
Please refer to our alternative product Catalog No. 91213

Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed overnight at 37°C. Individual optimization may be required.

For c-Myc, we also offer AbFlex® c-Myc Recombinant Antibody (rAb).  For details, see Catalog No. 91213.

Immunogen

This c-Myc antibody was raised against a synthetic peptide corresponding to amino acid residues 9-22 of human c-Myc; it recognizes a 65 kDa form of c-Myc.

Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

 

c-Myc antibody (65 kDa form) tested by Western blot.
Detection of c-Myc by Western blot analysis. A 65 kDa form of the transcription factor c-Myc is detected in nuclear extracts derived from 3T6 Swiss albino cells using c-Myc rabbit polyclonal antibody at a 1:1,000 dilution (lane 1). The signal is eliminated when the c-Myc immunizing peptide is added to the reaction mixture (lane 2).

Background

c-Myc is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.

What’s in our c-Myc Toolbox?

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting