SALL4 antibody (pAb)
RRID: AB_2793408 |
Aliases: Spalt Like Transcription Factor 4, DRRS, HSAL4, ZNF797, dJ1112F19.1 |
Catalog No: 39957 | Format: 100 µl | $500 | Buy |
Catalog No: 39958 | Format: 10 µl | $125 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
IP: 10 µl per IP
WB*: 1:500 - 1:2,000 dilution
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This SALL4 antibody was raised against a synthetic peptide derived from the N-terminus of human SALL4.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Background
SALL4 (Sal-like protein 4) is a zinc-finger transcription factor required for the maintenance of embryonic stem cell pluripotency. SALL4 activates the expression of Oct4 and reduction in the levels of SALL4 eliminates the ability of ES cells to self-renew and causes differentiation. SALL4 mutations are associated with the developmental disorders IVIC syndrome and Duane-radial ray syndrome.