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Contents
2 x 100 µg of WI-38 nuclear extract at 2.5 µg/µl.
Background
WI-38 nuclear extract was prepared from a cell culture of the human lung fibroblast WI-38 cell line. The WI-38 cell line was originally derived from lung tissue obtained from a Caucasian female fetus of 3 months gestation. The WI-38 cell line was the first normal diploid human cell line to be continuously cultivated. WI-38 cells are known to have the broadest viral spectra of any cell population that has been tested and are frequently used in research to isolate viruses, such as rhinovirus, and for the production of viral vaccines, including rubella, rabies, and hepatitis A. These cells have a limited replicative lifespan of approximately 50 population doublings after which they enter a state of irreversible growth arrest known as replicative senescence. WI-38 cells are most commonly used in research in studies of viral production, replicative senescence, and as normal controls.
Application Notes
WI-38 nuclear extract is specifically recommended for studies related to viral production and replicative senescence, and as normal diploid controls.
Extract Origin
Human normal lung fibroblast
Extract Composition
WI-38 nuclear extract was collected in Lysis Buffer. The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.
Quality Control
Each lot has been tested for pCREB activation by using TransAM® pCREB Kits. The signal intensity for pCREB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 2 when using TransAM® pCREB. This ratio may vary depending on the basal level of pCREB activation in a given cell type.
Storage
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
