Progesterone Receptor antibody (mAb)

RRID: AB_2793476
Clone: 6A
Aliases: PGR, PR, NR3C3
Catalog No: 61023 Format: 100 µg Discontinued

Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
100 kDa
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

Application Notes


This product has been discontinued
Validated Applications:
ChIP: 0.5 - 2 µg/ml dilution

Published Applications:
ICC/IF
IHC
WB
See references for more information. Individual optimization may be required.

Immunogen

This Progesterone Receptor antibody was raised against a peptide within the C-terminus of human Progesterone receptor.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Progesterone Receptor antibody (mAb) tested by ChIP.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 4.5 million treated MCF-7 breast cancer cells and 4 µl of Progesterone Receptor antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme, which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Background

Progesterone Receptor (PR, NR3C3) is a nuclear receptor that binds the steroid hormone progesterone, translocates into the nucleus and activates transcription.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Progesterone Receptor antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting