Histone H3K9ac antibody (mAb)

Application Notes

Applications Validated by Active Motif:
ChIP: 2 - 5 µg per ChIP
ChIP-Seq: 5 µg each
WB: 0.2 - 2 µg/ml dilution
DB: 0.2 - 2 µg/ml dilution
CUT&Tag: 1 µg per 50 µl reaction

NGS-QC^® certification: this antibody has been processed by the NGS-QC^® generator. For additional details, click here.

For Histone H3K9ac, we also offer AbFlex^® Histone H3K9ac Recombinant Antibody (rAb). For details, see Catalog No. 91103.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR, ChIP-Seq
ICC/IF
IHC
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

The Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human Histone H3.

Buffer

Purified IgG in 63mM Trisodium Citrate, 70mM Sodium Phosphate Tribasic, 301mM Sodium Chloride, 30% Glycerol, and 0.035% Sodium Azide. Sodium Azide is highly toxic.

*Due to the high levels of NA+ in the antibody storage buffer, precipitation might be observed. Incubate the antibody tube at 37°C and mix prior to use.

References

Background

Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of Lys9 is associated with trascriptional activation of genes.

qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT^® High Sensitivity Kit or our magnetic bead-based ChIP-IT^® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9ac antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

• ChIP = Chromatin Immunoprecipitation;
• ChIP = ChIP Sequencing;
• CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
• CUT&Tag = Cleavage Under Targets and Tagmentation;
• DB = Dot Blot;
• ELISA = Enzyme-linked Immunosorbent Assay;
• EMSA = Electrophoretic Mobility Shift Assay
• FC = Flow Cytometry;
• ICC = Immunocytochemistry;
• IF = Immunofluorescence;
• IHC = Immunohistochemistry;
• IP = Immunoprecipitation;
• MeDIP = Methyl-DNA Immunoprecipitation;
• NEU = Neutralizing;
• WB = Western Blotting