Supt3h antibody (mAb)
RRID: AB_2614996 |
Clone: 1SP-2C10 |
Aliases: Suppressor of Ty 3 homolog (S. cerevisiae), SPT3, SPT3L |
Catalog No: 61343 | Format: 100 µg | Discontinued | |
Catalog No: 61344 | Format: 10 µg | Discontinued |
Applications
Application Notes
This product has been discontinued:
Please see our related product - Supt16H antibody (pAb) Catalog No. 61441
Published Applications:
ChIP
IP
WB
See references for more information. Individual optimization may be required.
Immunogen
This antibody was raised against a peptide corresponding to amino acid residue(s) 10-27 of human Supt3h.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Background
Supt3h (suppressor of Ty 3 homolog (S. cerevisiae)) is a probable transcriptional activator and functions as a component in multiple histone acetyltransferase (HAT) complexes such as STAGA (SPT3-TAF-GCN5 acetylase), PCAF (p300/CBP associated factor), and TFTC (TBP-free TAF complex).
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Supt3h antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting