Supt3h antibody (mAb)

RRID: AB_2614996
Clone: 1SP-2C10
Aliases: Suppressor of Ty 3 homolog (S. cerevisiae), SPT3, SPT3L
Catalog No: 61343 Format: 100 µg Discontinued
Catalog No: 61344 Format: 10 µg Discontinued

Antibody Type:
Monoclonal
Isotype:
IgG1, k
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
38 kDa
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated

Application Notes


This product has been discontinued:
Please see our related product - Supt16H antibody (pAb) Catalog No. 61441


Published Applications:
ChIP
IP
WB
See references for more information. Individual optimization may be required.

Immunogen

This antibody was raised against a peptide corresponding to amino acid residue(s) 10-27 of human Supt3h.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Background

Supt3h (suppressor of Ty 3 homolog (S. cerevisiae)) is a probable transcriptional activator and functions as a component in multiple histone acetyltransferase (HAT) complexes such as STAGA (SPT3-TAF-GCN5 acetylase), PCAF (p300/CBP associated factor), and TFTC (TBP-free TAF complex).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Supt3h antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting