Histone H2AQ104me1 antibody (pAb)

RRID: AB_2793698
Catalog No: 61605 Format: 100 µl $500 Buy
Catalog No: 61606 Format: 10 µl $125 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
14 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Dot Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1:500 - 1:1,000 dilution

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Immunogen

This antibody was raised against a peptide containing human histone H2AQ104me1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 

Histone H2AQ104me1 antibody (pAb) tested by Western blot.
Histone H2AQ104me1 detection by Western blot. The analysis was performed using 30 µg of HeLa nuclear cell extract and Histone H2AQ104me1 antibody at a 1:500 dilution.

Histone H2AQ104me1 (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2AQ104me1 pAb for monomethyl-glu104 of histone H2A. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000.
    Lane 1: Unmodified glutamine 104 of H2A peptide.
    Lane 2: Monomethylated glutamine 104 of H2A peptide.

Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Methylation on glutamine 104 has been implicated in RNA Pol-I transcriptional regulation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting