Recombinant SUV420H2 protein

Catalog No: 81013 Format: 20 µg $475 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

SUV420H2 (Suppressor Of Variegation 4-20 Homolog 2, also known as KMT5C) is a histone methyltransferase that specifically trimethylates nucleosomal histone H4 on lysine 20 (H4K20). H4K20me3 is a histone marker that represents epigenetic transcriptional repression. SUV420H2 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H2 is targeted to histone H3 via its interaction with RB1 family proteins.

Application Notes

Recombinant SUV420H2 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 2 µg Recombinant Nucleosomes (H3.1) (Cat. 31466) were incubated with 0 µg (-), 0.1 µg (+), 0.2 µg (++), 0.4 µg (+++) SUV420H2 protein, in 30 µl reaction containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 3 hr at RT. Products run on a 12.5% SDS-PAGE gel were detected with anti-H4K20me2 (Cat. 39540) or anti-H4K20me3 (Cat. 39672). SUV420H2 only was used as negative control.

Protein Details

Recombinant SUV420H2 protein was expressed in E. coli cells as full length protein (accession number NP_116090.2) and contains an N-terminal GST tag with an observed molecular weight of 78.4 kDa.

 

Recombinant SUV420H2 protein

10% SDS-PAGE Coomassie staining

MW: 78.4 kDa

Purity: > 60% 

Recombinant SUV420H2 activity assay
Recombinant Nucleosomes (H3.1) in various amounts were incubated with SUV420H2 protein. Reaction products were run on SDS-PAGE gel and detected with anti-H4K20me2 or anti-H4K20me3. The activity of SUV420H2 with nucleosomes as substrate is better than with histone octamers as substrate. 

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.