Recombinant METTL13 protein

Catalog No: 81024 Format: 20 µg $490 Buy
Catalog No: 81724 Format: 1 mg $4,050 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

METTL13 (methyltransferase-like 13, is known as feat, CGI-01, KIAA0859, 5630401D24Rik) contains a conserved S-adenosylmethionine-binding motif, It is a potential SAM-dependent methyltransferase.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant METTL13 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_057019.3) with an N-terminal FLAG tag. The molecular weights of METTL13 is 80 kDa.

 

Recombinant METTL13 protein gel.

10% SDS-PAGE Coomassie staining

MW: 80 kDa

Purity: ≥87%

MTase-Glo assay for METTL13 methyltransferase activity
1 μM EEF1A1 protein and 1 μM SAM was incubated with different concentrations of METTL13 protein in 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation.

MTase-Glo assay for METTL13 methyltransferase activity
1 μM EEF1A1 protein and 1 μM SAM was incubated with different concentrations of METTL13 protein in 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -20°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.