Recombinant STAT3 protein

Catalog No: 81095 Format: 20 µg $450 Buy
Catalog No: 81895 Format: 1 mg $4,050 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

STAT3 (Signal Transducer And Activator Of Transcription 3), also known as APRF (Acute-Phase Response Factor) or ADMIO, HIES, is a signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, STAT3 can recruit coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. Deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. STAT3 can bind to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. It also acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells. It is involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. 

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant human STAT3 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_644805.1) with an N-terminal FLAG tag. The molecular weight of the protein is 89.2 kDa.

 

Recombinant STAT3 protein gel
10% SDS-PAGE Coomassie staining
MW: 89.2 kDa
Purity: >90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.