AbFlex® MAZ antibody (rAb)

RRID: AB_2793803
Aliases: MYC Associated Zinc Finger Protein, Transcription Factor Zif87, ZNF801
Catalog No: 91205 Format: 100 µg $385 Buy
Catalog No: 91206 Format: 10 µg $85 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
55 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1 - 4 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39935.

Immunogen

This antibody was raised against human Maz protein [MYC-associated zinc finger protein], NP_002374.

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> MAZ antibody (rAb) tested by Western blot.

AbFlex® MAZ antibody (rAb) tested by Western Blot.
10 µg MCF7 cell nuclear extract was run on SDS-PAGE, and probed with 2 µg/ml AbFlex MAZ antibody. MW: 55 kDa

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. MAZ (Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. MAZ binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. MAZ also participates in skeletal muscle development through the activation of several skeletal muscle-specific genes. MAZ has also been reported to drive tumor-specific expression of PPAR-gamma in breast cancer cells.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting