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TransAM® NFκB Assay Kits

dna-binding ELISAs for activated NFkB transcription factors

TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Active Motif offers multiple TransAM formats in order to meet your research needs when studying NFkB. In addition, the NFkB respone element is available as a pre-cloned transfection ready luciferase reporter vector on the LightSwitch™ Reporter System page under Synthetic Response Element Collection, as well as three different validated NFkB pathway reporter stable cell lines, containing either the NFkB response element or the IL-8 or NFKB2 endogenous promoters stably integrated into HT1080 cells.

Which TransAM® Kit is right for you?

The table below gives a brief overview of the targeted transcription factor(s) and different features of each TransAM Kit format with links to obtain more information.

Kit Format Features Targets
Colorimetric TransAM® NFkB
  • 96-stripwell plate pre-coated with consensus NFkB binding site
  • Works with as little as 2 µg nuclear or 4 µg whole-cell extract

TransAM® NFkB p50

TransAM® NFkB p52

TransAM® NFkB p65

TransAM® NFkB Family

Chemiluminescent TransAM® NFkB Chemi
  • 96-stripwell plate pre-coated with consensus NFkB binding site
  • Works with as little as 0.2 µg nuclear or 0.4 µg whole-cell extract

TransAM® NFkB p50 Chemi

TransAM® NFkB p52 Chemi

TransAM® NFkB p65 Chemi

Flexi Colorimetric TransAM® Flexi NFkB
  • Design your own biotinylated oligos to study variant transcription factor-binding sites
  • Bind biotinylated oligos and sample in solution, then transfer to streptavidin coated 96-stripwell plate

TransAM® Flexi NFkB p50

TransAM® Flexi NFkB p65

TransAM® Flexi NFkB Family

 

NFkB Transcription Factor Info

The transcription factor NFkB (NF kappa B, NF-kB, or nuclear factor kB) is widely studied due to its implication in the regulation of genes that control inflammation, cell proliferation and cell survival. NFkB is comprised of homo- or heterodimers of different subunits. These subunits are members of the structurally related Rel family of transcription factors. Five different Rel proteins (also called Rel/NFkB proteins) have been identified: p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel.

p65 (RelA), RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with RelA, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFκB signaling pathway.

Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IκB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IκB proteins. This releases NFκB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.

For additional information on transcription factor NFkB, please click here.

The canonical and non-canonical NFκB cell signaling pathways 
 The canonical and non-canonical NFκB cell signaling pathways