Recombinant PPARα protein

Aliases: PPAR, NR1C1, hPPAR, PPARalpha
Catalog No: 31125 Format: 10 µg $450 Buy

Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Peroxisome Proliferator-Activated Receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: α, δ and ϒ. PPARs bind to peroxisome proliferator response elements (PPREs) as heterodimers with the retinoid X receptor (RXR).

Application Notes

Recombinant PPARα is suitable for DNA and protein-protein interaction assays. 20 ng is sufficient for gelshift assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~52 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Protein Details

Recombinant PPARα is isolated from an E. coli strain that carries the coding sequence of the human PPARα under the control of a T7 promoter (accession number NM 005036). The purified recombinant protein has an N-terminal His-Tag and is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.