Recombinant PRDM9 (191-414) protein

Aliases: PFM6, MSBP3, ZNF899, MEISETZ
Catalog No: 31510 Format: 20 µg $450 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

PRDM9 (PR Domain Containing 9) is a Histone methyltransferase that specifically trimethylates Lys-4 of histone H3 during meiotic prophase and is essential for proper meiotic progression. PRDM9 has the ability to mono- and dimethylate Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase (By similarity).

Application Notes

Recombinant PRDM9 is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 50 mM TrisCl, pH 8.5, 30 mM NaCl, 5% glycerol, 2 mM DTT, 5, 25, and 50 μM H3 (1-24) peptide, 100 nM Recombinant PRMT5 protein at room temperature.

Protein Details

Recombinant PRDM9 protein contains amino acids 191 - 414 of the human PRDM9 (accession number NP_064612.2) with a GST-Tag and expressed in E.coli with a molecular weight of 25.6 kDa.

 

Recombinant PRDM9 protein gel.
PRDM9 protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue.

Recombinant PRDM9 protein activity assay.
PRDM9 protein was used in an HMT assay to determine enzyme acticvity. Activity was detected by MALDI-TOF.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.