Recombinant Histone H3K36me3 (MLA)

Aliases: H3K36me3, Histone H3, methylated lysine analog
Catalog No: 31580 Format: 100 µg $320 Buy
Catalog No: 31980 Format: 1 mg $935 Buy

Request a quote for a bulk order Request Quote


Expressed In: E. coli Protein Species: Human

Methylated Lysine Analog

Recombinant Histone H3K36me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ϒ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

Recombinant Histone H3K36me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine Υ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

Application Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post- translational modi cations, as substrates for histone modifiation enzymes, or to generate chromatin in vitro.

Protein Details

Recombinant human Histone H3 trimethyl Lys36 (H3K36me3) (accession number NP_003520.1) was expressed in E. coli cells and contains two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110). Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). The molecular weight of the recombinant histone is 15,285 Daltons. The recombinant histone is >92% pure by SDS-PAGE.
Protein Sequence (a.a. substitutions in italics): ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVCKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSSAVM ALQEASEAYL VGLFEDTNLA AIHAKRVTIM PKDIQLARRI RGERA

References

This product was used in the following publications:
Nucleic Acids Res. (2019). Jun 5. pii: gkz493. PMID: 31165872. (Histone Demethylation Assay)

 

Recombinant Histone H3K36me3 (MLA) gel. Histone H3K36me3 (MLA) was run on a 12.5% SDS- PAGE gel and stained with Coomassie Blue.

Mass Spec analysis for Recombinant Histone H3K36me3 (MLA) gel.
Mass Spec analysis shows that almost all of histones. are K36me3 histones.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.