Recombinant METTL8 protein, GST-Tag

Catalog No: 81106 Format: 50 µg $490 Buy
Catalog No: 81806 Format: 1 mg $3,000 Buy

Request a quote for a bulk order Request Quote


Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

METTL8 (methyltransferase-like 8, also known as TIP) contains a conserved S-adenosylmethionine-binding motif. It is a potential SAM-dependent methyltransferase.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Full length recombinant METTL8 (accession number NP_079046.2) was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of the protein is 73.3 kDa.

 

Recombinant METTL8 protein, GST-Tag
10% SDS-PAGE Coomassie staining
MW: 73.3 kDa
Purity: ≥90%

MTase-Glo assay for METTL8 methyltransferase activity
400 ng total RNA and 1 μM SAM was incubated with different concentrations of METTL8 protein in 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/μL RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation.

MTase-Glo assay for METTL8 methyltransferase activity
400 ng total RNA and 1 μM SAM was incubated with different concentrations of METTL8 protein in 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/μL RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.